DAYA HAMBAT SARI UMBI BAWANG MERAH(Allium ascalanicum. L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Pseudomonas aeruginosa ISOLAT TONSILITIS

Irawan, Arif Ade, G1C216113 (2017) DAYA HAMBAT SARI UMBI BAWANG MERAH(Allium ascalanicum. L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Pseudomonas aeruginosa ISOLAT TONSILITIS. Sarjana (S1/D4) thesis, Universitas Muhammadiyah Semarang.

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Abstract

Tujuan penelian untuk mengetahui dan menganalisis pengaruh konsentrasi 10%, 20%, 30%, 40% dan 50% b/v dalam menghambat pertumbuhan bakteri S. aureus dan P. aeruginosa. Jenis penelitian ini eksperimental dengan design post test with control. Tahapan penelitian diawali dengan kultifikasi (peremajaan bakteri) S. aureus dan P. aeruginosa. Pembuatan sari umbi bawang merah dengan cara di blender dengan perbandingan sesuai konsentrasi. Sari umbi bawang dibuat dan disaring menggunakan kasa steril dan pelarut yang digunakan adalah aquades steril. Metode pengujian menggunakan difusi sumuran. Hasil menunjukan diameter sari umbi bawang merah dengan konsentrasi 10%, 20%, 30%, 40% dan 50% b/v dapat menghambat pertumbuhan bakteri S. aureus dengan rerata berurutan 11,0 mm, 12,0 mm, 13,0 mm, 14,0 mm dan 15,0 mm, sedangkan pada sampel P. aeruginosa tidak tebentuk zona hambat. Hasil uji Anova pada zona hambat bakteri S. aureus signifikansi dengan nilai p=0,000. Kata kunci : Daya hambat, P. aeruginosa, Sari umbi bawang merah, S. aureus. This research is aims to know and analysis the influence of 10%, 20%, 30%, 40% and 50% w/v concentration in inhibiting the growth of S. aureus and P. aeruginosa bacteria. This type of research is experimental with design post test with control. The research stages were initiated by the cultivation of S. aureus and P. aeruginosa. Onions essence made by blended with comparison according to concentrations. Onions essence was filtered using sterile gauze and solvent used is sterile aquades. Test method using diffusion well. The results showed the diameter of the onion essence with concentrations of 10%, 20%, 30%, 40% and 50% b/v can inhibit the growth of S. aureus bacteria with average are 11.0 mm, 12.0 mm, 13.0 mm, 14.0 mm and 15.0 mm, while in the sample P. aeruginosa did not form inhibit zone. Anova test in the S. sureus bakterial inhibition zone results of significance with p value 0.000 < 0,05. Keywords: Inhibitory power, P. aeruginosa, Onion, S. aureus.

Item Type: Thesis (Sarjana (S1/D4))
Contributors Thesis: 098/D4Ana/XII/2017
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 14 Dec 2017 04:27
Last Modified: 13 Apr 2018 06:37
URI: http://repository.unimus.ac.id/id/eprint/1135

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