PROFIL TOTAL PROTEIN Aedes sp. DAERAH NON ENDEMIK DEMAM BERDARAH KECAMATAN MIJEN KOTA SEMARANG

Haryanti, Siti, G1C013038 (2017) PROFIL TOTAL PROTEIN Aedes sp. DAERAH NON ENDEMIK DEMAM BERDARAH KECAMATAN MIJEN KOTA SEMARANG. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Penyakit Demam Berdarah (DBD) merupakan penyakit yang ditimbulkan oleh virus dengue yang ditularkan melalui gigitan Aedes sp dan tersebar luas pada lokasi desa maupun perumahan. Di Indonesia merupakan salah satu masalah kesehatan yang memiliki resiko besar terjangkit penyakit ini. Tahun 2014 Kota Semarang dengan penderita 14,7% kasus di Jawa Tengah. Semarang terbagi menjadi 2 wilayah yaitu dataran tinggi dan rendah. Mijen merupakan daerah non endemik. Profil protein merupakan penentuan kekerabatan antar makhluk hidup dan dipengaruhi oleh kondisi geografis serta tempat. Tujuan penelitian ini untuk mengetahui profil total protein Aedes sp. pada daerah non endemik Kecamatan Mijen Kota Semarang. Analisis dilakukan pada 2 lokasi yaitu lokasi desa dan lokasi perumahan menggunakan Elekroforesis SDS-PAGE. Subjek dan objek penelitian telur Aedes sp. yang berasal dari daerah non endemik Kecamatan Mijen Kota Semarang ditetaskan dan dipelihara menjadi nyamuk dewasa dan dibaca profil proteinnya. Metode penelitian Deskriptif. Hasil analisis profil protein Aedes sp. menunjukkan pada Lokasi Desa menunjukkan 22 pita protein dengan 6 pita protein mayor dan 16 pita protein minor dengan berat molekul antara 165 kDa sampai 14 kDa, Sedangkan Lokasi Perumahan menunjukkan 15 pita protein dengan 2 pita protein mayor dan 13 pita protein minor serta berat molekul antara 165 kDa sampai 17,5 kDa. Kata Kunci : Aedes sp, Profil Total Protein, Daerah Non Endemik DBD Dengue Fever Disease (DHF) is a disease caused by dengue virus that is transmitted through Aedes sp mosquito bites and is widespread on the location of the village and housing. In Indonesia is one of the health problems that have a great risk of contracting this disease. In 2014 the city of Semarang with 14.7% of cases in Central Java. Semarang divided in 2 region high and low. Mijen is non endemic region. The protein profil is determine of relationship between living creatures and geographic conditions as well as place can influence protein profile. This experiment to know total profile protein Aedes sp. non endemic hemorrhagic fever in mijen district of Semarang city. Analyze worked in 2 locations: village location and housing location using SDS-PAGE Elecrophoresis method. Eksperiment subject and object : Aedes sp. egg from the non endemic areas of Mijen sub district Semarang City the growth become adult mosquito and read protein profile. Method: Descriptive Method. The analyze results Aedes sp. show: Village location shows 22 protein bands with 6 bands major protein and 16 bands minor protein with a molecular weight of between 165kDa to 14 kDa , while Housing locations shows 15 protein bands with 2 bands major protein and 13 bands minor protein with molecular weight between 165kDa to 17,5 kDa. Keywords: Aedes sp, Total Protein Profile, Non-Endemic DHF

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