DAYA HAMBAT EKSTRAK ETANOL KAYU SECANG (Caesalpinia sappan L.) TERHADAP PERTUMBUHAN Klebsiella pneumonia

Riskayanti, G1C216114 (2017) DAYA HAMBAT EKSTRAK ETANOL KAYU SECANG (Caesalpinia sappan L.) TERHADAP PERTUMBUHAN Klebsiella pneumonia. Undergraduate thesis, Universitas Muhammadiyah Semarang.

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Abstract

Kayu secang (Caesalpinia sappan L.) secara tradisional dapat digunakan untuk mengobati diare, disentri, batuk darah pada TBC, muntah darah, sifilis, malaria, tetanus, pembengkakan (tumor) dan nyeri serta mengandung senyawa brasilin, tanin, saponin, flavonoid, alkaloid dan minyak atsiri yang berfungsi sebagai antibakteri. Tujuan penelitian untuk mengukur dan menganalisis perbedaan konsentrasi 15%b/v, 30%b/v, 45%b/v dan 60%b/v ekstrak etanol kayu secang yang paling baik dalam menghambat pertumbuhan K.pneumoniae. Desain penelitian adalah eksperimen dengan objek penelitian kayu secang dan K. pneumoniae. Tahapan penelitian diawali dengan peremajaan bakteri K. pneumoniae, pembuatan ekstrak etanol kayu secang dengan metode maserasi selama 72 jam menggunakan etanol 96% dan pengujian menggunakan metode difusi sumuran. Hasil menunjukkan ekstrak etanol kayu secang konsentrasi 15%b/v, 30%b/v, 45%b/v dan 60%b/v dapat menghambat dengan rata-rata diameter daya hambat berturut-turut 16 mm, 16,37 mm, 16,87 mm dan 17,37 mm. Hasil uji One Way Anova menunjukkan adanya perbedaan bermakna antara konsentrasi ekstrak etanol kayu secang terhadap pertumbuhan bakteri K. pneumoniae. Semakin tinggi konsentrasi ekstrak etanol kayu secang maka semakin besar daya hambat terhadaap pertumbuhan bakteri K. pneumoniae. Kata Kunci : Daya hambat, Ekstrak etanol kayu secang, Klebsiella pneumoniae. Secang wood (Caesalpinia sappan L) traditionally can be used to treat diarrhea, dysentry, coughing blood in TBC, vomiting blood, syphilis, malaria, tetanus, swelling (tumor) and sore. Contain compound of brasilin, tannin, saponin, flavonoid, alkaloid and essential oils serve as antibacterials. The purpose of the research was to know and to analyze the effect of different concentration of 15%b/v, 30%b/v, 45%b/v and 60%b/v wich the best of ethanol extract of wood secang in inhibiting against of growth K. pneumoniae. The design of the research is using experiment, with object research are secang wood and K. pneumoniae. The research phose begins with rejuvenation of bacteria K. pneumonia preparation of ethanol extract of wood secang with maseration method for 72 hours using ethanol 96% and testing using well diffusion method. The result showed concentration of ethanol extracts of secang wood 15%w/v, 30%w/v, 45%w/v and 60%w/v can inhibited by the average of potency inhibitor diameter consecutive 16 mm, 16,37 mm, 16,78 mm and 17,37 mm. The result of One Way Anova test showed there’s difference between ethanol extract of secang wood againts growth of bacteria K. pneumoniae. The highen concentration of secang wood the greaten the inhibitor effect on growth of bacteria K. pneumoniiae. Keyword: Ethanol Extract Of Wood Secang, Klebsiella pneumoniae, Potency inhibitory.

Item Type: Thesis (Undergraduate)
Contributors Thesis: 228/D4Ana/II/2018
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 28 Dec 2017 07:39
Last Modified: 10 Apr 2018 04:10
URI: http://repository.unimus.ac.id/id/eprint/1262

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