SENSITIVITAS DAN SPESIFISITAS METODE ELISA TERHADAP METODE ELFA PADA PEMERIKSAAN HEPATITIS B

Indiati, Ina Restin, G1C216067 (2017) SENSITIVITAS DAN SPESIFISITAS METODE ELISA TERHADAP METODE ELFA PADA PEMERIKSAAN HEPATITIS B. Undergraduate thesis, Universitas Muhammadiyah Semarang.

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Abstract

Pemilihan metode yang tepat akan bermanfaat untuk pelayanan laboratorium khusus pemeriksaan hepatitis. Sensitivitas dan spesifisitas metode perlu menjadi perhatian agar pelayanan efektif dan efisian dapat dilaksanakan. Metode ELISA yang saat ini telah banyak ditinggalkan masih dapat dipakai sebagai alternatif metode yang lebih ekonomis, meskipun metode ELFA telah banyak digunakan. Penelitian ini bertujuan untuk mengetahui sensitivitas dan spesifisitas metode ELISA terhadap metode ELFA pada pemeriksaan hepatitis B. Penelitian ini dilakukan terhadap 90 sampel pasien rawat inap dan rawat jalan yang berkunjung ke laboratorium RSUD Prof. Dr. W. Z. Johannes Kupang. Sampel darah vena sebanyak 3 ml dalam Vacutainer diambil serumnya dan dilakukan pemeriksaan hepatitis dengan metode ELISA dan ELFA. Digunakan uji diagnostik menggunakan tabel 2x2 untuk menganalisa sensitivitas dan spesifisitas. Hasil penelitian ini menunjukkan sensitivitas metode ELISA terfhadap ELFA adalah 98,36% dan spesifisitasnya 100% menunjukkan kategori sangat baik. Kata kunci: hepatitis B, sensitivitas ,sensitifitas, ELISA,ELFA. Selection of appropriate methods will be useful for specialized laboratory services of Hepatitis examination. The sensitivity and specificity of the method should be of concern in order for effective and efficient services to be implemented. The currently abandoned ELISA method can still be useda an alternative to more econimical methodsalthough ELFA has been widely used. This research aims to determine the sensitivity and specificity of the ELISA method against RLFA method on Heptitis B examination. This research was conducted on 90samples of inpatients and outpatients who visited the laboratory of Prof. DR. W. Z. Yohanes Kupang. 3 cc venous blood sample in vacuta was taken serum and Hepatitis examination by ELISA and ELFA method. Using 2x2 tables to analyze sensitivity and specificity. The results of this study showed that ELISA’s ELFA sensitivity to ELFA was 98,36% and its 100% accuracy showed very good category of in. Keywords: Hepatitis B, sensitivity, specificity, ELISA, ELFA.

Item Type: Thesis (Undergraduate)
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 18 Jan 2018 02:49
Last Modified: 18 Jan 2018 02:49
URI: http://repository.unimus.ac.id/id/eprint/1406

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