SABUN CUCI PIRING (DISHWASHING SOAP) SEBAGAI PENGGANTI XYLOL PADA PROSES DEPARAFINASI PENGECATAN IMUNOHISTOKIMIA HER2

Pranata, Ardi, G1C013034 (2017) SABUN CUCI PIRING (DISHWASHING SOAP) SEBAGAI PENGGANTI XYLOL PADA PROSES DEPARAFINASI PENGECATAN IMUNOHISTOKIMIA HER2. Undergraduate thesis, Universitas Muhammadiyah Semarang.

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Abstract

Breast cancer has three molecular biomarker that is estrogen receptor (ER), progesterone receptor (PR), and HER2 adopted as a routine examination that played in determining the prognosis and predictive cancer. Generally, the biomarker examination conducted by immunohistochemical methods. The use of xylol in HER2-staining deparaffinization process replaced with dishwashing soap. Purpose of the research was to described the result of staining using 1.5%, 2%, 2.5% of dishwashing soap and analysis difference of staining result compared with xylol. This research was experimental with cross-sectional approach. Sample of the research using HER2-positive breast cancer with score +3 from single patient. Methode of HER2-staining used Strep(Avidin) Biotin Complex technique. HER2-staining used 1,5%, 2%, 2,5% of dishwashing soap and xylol as control. The result of HER2-staining used xylol as deparaffinization agent showed obtained score +3, used 1,5% dishwashing soap obtained score +2, while 2% and 2.5% dishwashing soap obtained score +3. There is a significant difference between xylol with dishwashing soap 1.5%. There was no significant difference between xylol with dishwashing soap 2% and 2.5%. Xylol on HER2 immunohistochemical staining deparaffinization process can be replaced with dishwashing soap 2%. Keyword: HER2, xylol, deparaffinization, dishwashing soap

Item Type: Thesis (Undergraduate)
Contributors Thesis: 060/D4Ana/XI/2017
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 22 Jan 2018 04:12
Last Modified: 13 Apr 2018 04:02
URI: http://repository.unimus.ac.id/id/eprint/1437

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