PENGARUH PENUNDAAN SAMPEL URIN pH ALKALI METODE KONVENSIONAL TERHADAP UNSUR ORGANIK SEDIMEN URIN

Tena, Heribertus Agustinus Bilo, G1C216066 (2017) PENGARUH PENUNDAAN SAMPEL URIN pH ALKALI METODE KONVENSIONAL TERHADAP UNSUR ORGANIK SEDIMEN URIN. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Suspension check sample urine pH alkaline will affect quality results examination to elements shaped microscopic sediment urine. Element organic sediment urine in urine with an alkaline pH (pH> 7, 5) and is dilute (hypotonic) will cause cells on sediment urine experience destruction (lysis) if do delay examination. This study aim early to determine the effect of time delay examination sample urine pH alkaline method conventional to element organic sediment urine. this study was conducted on 6 samples of hospitalized patients who do morning urine examination in the laboratory of Prof. Hospital Dr. Z. W. Johannes Kupang. urine sample morning 10 mL examination sediment urine using conventional methods. Used repeat ed Anova test for sediment of leucocytes and epithelial and Friedman's test erythrocyte sediment using SPSS software to analyze the effect of delay urine sample pH alkaline to element organic sediment urine at a time delay of 1 hour, 2 hours and 3 hours at room temperature. Anova test results repeat ed and test Friedman show delay time examination urine sample pH alkaline no influence results examination element organic urine sediment leukocytes (p>0.05), but has influence results examination element organic urine sediment erythrocytes and epithelium (p<0.05) until with a 3 hour delay. This study shows no there is influence delay time of inspection sample urine pH alkaline method conventional to element organic urine sediment leukocytes, however has influence results examination sedimentary organic elements urine erythrocytes and epithelium to delay 3 hours at temperature room. Keywords: Delay Examination, Conventional, Elements Organic Sediment Urine. Penundaan pemeriksaan sampel urin pH alkali akan mempengaruhi kualitas hasil pemeriksaan terhadap unsur-unsur berbentuk mikroskopis sedimen urin. Unsur organik sedimen urin dalam urin dengan pH alkali (pH>7,5) dan bersifat encer (hipotonik) akan menyebabkan sel-sel pada sedimen urin mengalami kerusakan (lisis) apabila dilakukan penundaan pemeriksaan. Penelitian ini bertujuan untuk mengetahui pengaruh penundaan waktu pemeriksaan sampel urin pH alkali metode konvensional terhadap unsur organik sedimen urin. Penelitian ini dilakukan terhadap 6 sampel pasien rawat inap yang melakukan pemeriksaan urin pagi di laboratorium RSUD Prof. Dr. W. Z. Johannes Kupang. Digunakan uji repeated anova untuk sedimen leukosit dan epitel dan uji Friedman untuk sedimen eritrosit dengan menggunakan software SPSS. Hasil uji repeated anova dan uji Friedman menunjukkan penundaan waktu pemeriksaan sampel urin pH alkali tidak mempengaruhi hasil pemeriksaan unsur organik sedimen urin leukosit (p>0.05), tetapi telah mempengaruhi hasil pemeriksaan unsur organik sedimen urin eritrosit dan epitel (p<0.05) sampai dengan 3 jam penundaan. Penelitian ini menunjukkan tidak ada pengaruh penundaan waktu pemeriksaan sampel urin pH alkali metode konvensional terhadap unsur organik sedimen urin leukosit, namun telah mempengaruhi hasil pemeriksaan unsur organik sedimen urin eritrosit dan epitel sampai penundaan 3 jam pada suhu ruangan. Kata kunci: Penundaan, konvensional, Unsur Organik Sedimen Urin.

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