Uji MIC (Minimum Inhibitory Concentration) Ekstrak Methanol Jamur Tiram Merah Muda (Pleurotus flabellatus) Terhadap Bakteri Multidrug Resistance Staphylococcus aureus Secara In Vitro

Saraswati, Dewi Kunthy, G0C015070 (2018) Uji MIC (Minimum Inhibitory Concentration) Ekstrak Methanol Jamur Tiram Merah Muda (Pleurotus flabellatus) Terhadap Bakteri Multidrug Resistance Staphylococcus aureus Secara In Vitro. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Infection disease is caused by pathogen microbe. Bacteria is one of pathogen microbe which cause infection. There is 42,1% infection disease especially infect the skin cause by Staphylococcus aureus (S.aureus). Purpose: To identify antibacterial activity from the methanol extract of P.flabellatus through Multidrug Resistance Staphylococcus aureus (MDR-S. aureus). To identify the MIC value from the extract. Method: The extraction process was maceration method by using a methanol solvent. The antibacterial activity was well diffusion method by using MHA and the MIC was micro-dilution method by using MHB. Results: Extract of the methanol extract of P. flabellatus showed polar constitution relative by 12.30%. The result of antibacterial activity using well method from the methanol extract of P.flabellatus towards MDR S. aureus was indicated by a diameter of slowing zone around of the well by 5.5 mm with the extract concentration as 1000 mg/mL. The extract which was examined showed antibacterial activity towards MDR S. aureus by 250 mg/mL of the MIC value. Conclusion: The the methanol extract of P.flabellatus is potentially to be an antibacterial towards MDR S. aureus. Keyword: Methanol extract, Pleurotus flabellatus, MDR, Staphylococcus aureus, MIC.

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