PROFIL PROTEIN IKAN BANDENG (Chanos-chanos) YANG DIRENDAM JERUK NIPIS (Citrus aurantifolia) BERBASIS SDS-PAGE

Sitti Nurjanna J., G1C217249 (2018) PROFIL PROTEIN IKAN BANDENG (Chanos-chanos) YANG DIRENDAM JERUK NIPIS (Citrus aurantifolia) BERBASIS SDS-PAGE. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Milkfish is one of potential animal protein because it is easy to digest but easily decomposes and has a fishy smell. Lime is one of the fruits that has a distinctive aroma to neutralize fishy smell, contain citric acid, ascorbic acid, and amino acids (tryptophan and lysine) which can cause denatured proteins. The purpose of this research was to see the protein profile of milkfish without soaking the lime solution and soaking the lime solution with a variation of concentration is 10%, 15%, and 20% and the variation of soaking time 5, 10, and 15 minutes. The protein profile of milkfish was analyzed using the SDS-PAGE 12% method. The results showed that the protein control bands consist of 22 protein bands (8 major bands and 14 minor bands), after soaking with concentration 10% for 5 and 10 minutes, showing 20 protein bands (7 major bands and 13 minor bands), for 15 minutes there are 19 protein bands (6 major bands and 13 minor bands). Soaking with concentration 15% for 5, 10, and 15 minutes in a row showing 20 protein bands (7 major bands and 13 minor bands), 19 protein bands (6 major bands and 13 minor bands), and 18 protein bands (6 major bands and 12 minor bands). Soaking with concentration 20% for 5 and 10 minutes, there are 19 protein bands (6 major bands and 13 minor bands), for 15 minutes it showing 17 protein bands (6 major bands and 11 minor bands). The higher the concentration of lime and soaking time of lime solution, the more protein is denatured in milkfish. Based on the results showed that citric acid, ascorbic acid and amino acids (tryptophan and lysine) in lime can break peptide bonds in fish so that the resulting protein subunits change. Milkfish, lime, protein profile, SDS-PAGE

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