KETAHANAN MORFOLOGI TELUR Soil Transmitted Helminths MENGGUNAKAN VARIASI KONSENTRASI FORMALIN

Wulandari, Novita, G1C218146 (2019) KETAHANAN MORFOLOGI TELUR Soil Transmitted Helminths MENGGUNAKAN VARIASI KONSENTRASI FORMALIN. Undergraduate thesis, Universitas Muhammadiyah Semarang.

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Abstract

ABSTRAK Cara menegakkan diagnosa STH dengan melakukan pemeriksaan tinja secara langsung dan sebaiknya tidak lebih dari 2 jam. Tetapi spesimen tinja dapat diawetkan dengan bahan pengawet. Pengawet yang biasa digunakan untuk mengawetkan feses salah satunya yaitu formalin konsentrasi 5-10%. Formalin memiliki kandungan formaldehida yang dapat mengawetkan telur STH selama ±12 bulan. Penelitian ketahanan morfologi telur STH dengan konsentrasi rendah perlu dilakukan agar penggunaan pengawet formalin dapat diminimalisir. Maka dari tujuan penelitian ini yaitu melihat adanya perubahan morfologi telur STH menggunakan variasi konsentrasi formalin. Penelitian ini merupakan penelitian Eksperimental Laboratorium. Penelitian ini dilakukan selama 21 hari dengan metode yang digunakan yaitu metode direct (langsung) dengan cara feses yang telah dicampurkan dengan pegawet formalin konsentrasi 0,1%, 0,2% dan 0,3%. Lalu diteteskan pada objeck glass cekung lalu ditutup dengan deck glass yang sebelumnya diberi vaselin dipinggiran deck glass setelah itu diperiksa dibawah mikroskop lensa 10x dan 40x. Hasil pengamatan dikategorikan kedalam utuh dan tidak utuh. Dari hasil yang diperoleh didapatkan adanya perubahan morfologi telur STH setelah pemberian pengawet formalin konsentrasi 0,1%, 0,2% dan 0,3% dengan waktu pengamatan selama 21 hari. Dari hasil tersebut dapat disimpulkan bahwa adanya perubahan morfologi telur STH setelah pemberian formalin dengan konsentrasi 0,1%, 0,2% dan 0,3% dengan waktu pengamatan pada hari ke-1, hari ke-7, hari ke-14 dan hari ke-21. Kata kunci : Soil transmitted Helminths, Ketahanan, formalin. VIABILITY EGGS OF Soil Transmitted Helminths USING A VARIATION THE CONCENTRATION OF FORMALIN Novita Wulandari1, Arya Iswara2, Tulus Ariyadi2 1. Study programs DIV of Health Analyst Faculty of Nursing and Health Science University of Muhammadiyah Semarang 2. Laboratory Of Parasitology, Faculty Of Health Nursing, University Of Muhammadiyah Semarang ABSTRAK How to enforce STH diagnostics by conducting fecal examination directly and should not be more than 2 hours. But the stool specimens can be preserved with preservatives. Preservatives commonly used to preserve the stool one of which is the formalin concentration of 5-10%. Formalin has a formaldehyde content that can preserve STH eggs for ± 12 months. Research on the morphology of STH eggs with low concentration should be done so that the use of formalin preservatives can be minimized. Therefore, the purpose of this research is to look at the changes in the morphology of STH eggs using variations in formalin concentrations. This research is experimental Research laboratory. This study was conducted for 21 days with the method used is direct method by means of feces that have been mixed with the personnel of formalin concentration of 0.1%, 0.2% and 0.3%. Then it was set in the concave glass objeck and closed with a glass deck previously given Vaseline rims glass decks after it was examined under the lens microscope 10x and 40x. The observation is categorized into intact and not intact. From the results obtained a morphological change of STH eggs after the administration of preservatives formalin concentrations of 0.1%, 0.2% and 0.3% with the observation time for 21 days. From the results it can be concluded that there is a change in the morphology of STH eggs after the administration of formalin with concentrations of 0.1%, 0.2% and 0.3% with the observation time on Day 1, day 7, day 14 and day 21. Keywords: Soil transmitted Helminths, Viability, formaldehyde.

Item Type: Thesis (Undergraduate)
Call Number: 001/D4Ana/I/2020
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 07 Feb 2020 08:10
Last Modified: 10 Feb 2020 03:37
URI: http://repository.unimus.ac.id/id/eprint/3519

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