PROFIL TOTAL PROTEIN BAKTERI ISOLAT EKTOPARASIT Bandicota sp. DENGAN METODE SDS-PAGE

SAHIDU,SARFIYANI, G1C218013 (2019) PROFIL TOTAL PROTEIN BAKTERI ISOLAT EKTOPARASIT Bandicota sp. DENGAN METODE SDS-PAGE. Undergraduate thesis, Universitas Muhammadiyah Semarang.

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Abstract

ABSTRAK Tikus wirok (Bandicota sp.) merupakan hewan pengerat yang sangat merugikan dan menjadi reservoir beberapa patogen pada manusia dan hewan. Tikus Bandicota Sp. dapat menularkan penyakit secara langsung melului gigitan ektoparasit atau melalui bakteri yang terdapat pada ektoparasit. Penelitian ini bertujuan untuk mengetahui profil total protein bakteri isolat ektoparasit Bandicota sp. yang ditemukan pada kawasan Perumahan Kini Jaya Kota Semarang dengan menggunakan metode SDS-PAGE. Pada hasil profil total protein bakteri isolat ektoparasit Bandicota sp. dengan metode SDS-PAGE ditemukan bakteri S.epidermidis yang memiliki 21 sub unit protein yang terdiri dari 8 sub unit mayor dan 13 sub unit minor. Berat molekul sub unit protein mayor adalah 10 kDa, 16,8 kDa, 37 kDa, 42 kDa, 44 kDa, 56 kDa, 75 kDa, dan 99 kDa. Berat molekul sub unit protein minor adalah 10 kDa, 16 kDa, 19 kDa, 21 kDa, 23 kDa, 30 kDa, 68 kDa, 73 kDa, 86 kDa, 96 kDa, 106 kDa, 113 kDa, dan 117 kDa. Kata Kunci : Bandicota sp., Ektoparasit, Bakteri, Profil Protein, SDS-PAGE PROFILE OF TOTAL PROTEIN BACTERIAL ISOLATE EKTOPARASIT Bandicota sp. WITH SDS-PAGE METHOD Sarfiyani Sahidu1, Sri Darmawati2, Aditya Rahman Ernanto2 1. Health Analyst Study Program Faculty of Nursing and Health, University of Muhammadiyah Semarang. 2. Molecular Biology Laboratory, Faculty of Nursing and Health, University of Muhammadiyah Semarang. ABSTRACT Wirok rat (Bandicota sp.) Is a rodent that is very harmful and is a reservoir of several pathogens in humans and animals. Bandicota sp. can transmit disease directly through ectoparasites bites or bacteria contained in ectoparasites. This study aim to analyze the total protein profile of the bacterial isolates from ectoparasites of Bandicota sp. In the Kini Jaya Housing complex in Semarang using SDS-PAGE method. The result of the total bacterial protein profile was found S.epidermidis with 21 sub units proteins consists of 8 major sub units and 13 minor sub units. The molecular weight of the major protein subunits is 10 kDa, 16.8 kDa, 37 kDa, 42 kDa, 44 kDa, 56 kDa, 75 kDa, and 99 kDa. The molecular weight of minor protein subunits is 10 kDa, 16 kDa, 19 kDa, 21 kDa, 23 kDa, 30 kDa, 68 kDa, 73 kDa, 86 kDa, 96 kDa, 106 kDa, 113 kDa and 117 kDa. Keywords: Bandicota sp., Ectoparasites, Bacteria, Protein Profile, SDS-PAGE

Item Type: Thesis (Undergraduate)
Call Number: 10/D4Ana/I/2020
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 13 Feb 2020 03:39
Last Modified: 13 Feb 2020 03:39
URI: http://repository.unimus.ac.id/id/eprint/3558

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