PERBEDAAN KADAR CARDIAC TROPONIN I (cTnI) DARAH EDTA SEGERA DIPERIKSA DENGAN SIMPAN 2 JAM

Risdaryanti, G1C218185 (2019) PERBEDAAN KADAR CARDIAC TROPONIN I (cTnI) DARAH EDTA SEGERA DIPERIKSA DENGAN SIMPAN 2 JAM. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

ABSTRAK Pemeriksaan kadar cardiac troponin I (cTnI) membutuhkan hasil dengan ketepatan dan ketelitian tinggi. Metode pemeriksaan cTnI flourescence immunoassay dengan alat Point of Care Testing (POCT) dengan waktu tunggu atau Turn Around Time (TATs) kurang dari 60 menit. Spesimen harus diproses dan dianalisis segera karena troponin jantung relatif tidak stabil dalam darah lengkap sehingga, apabila disimpan harus dibekukan. Penyimpanan dalam suhu ruang hingga 2 jam menyebabkan rusaknya enzim lizozim, peningkatan pH sehingga kadar cTnI mengalami penurunan. Persyaratan spesimen untuk troponin adalah plasma bebas hemolisis. Sampel dapat didinginkan atau dibekukan apabila tidak segera diperiksa. Penelitian bertujuan mengetahui perbedaan kadar cTnI darah EDTA segera diperiksa dan simpan 2 jam. Jenis penelitian analitik, desain cross sectional. Penelitian dilakukan di RSUD dr Soewondo Kendal pada Juni-Juli 2019. Sampel penelitian sebanyak 16 berasal dari pasien IGD dengan gejala IMA. Pemeriksaan menggunakan POCT cTnI. Hasil penelitian kadar cTnI darah EDTA segera diperiksa 0,04-9,80 ng/mL, rerata 5,41 ng/mL. Kadar cTnI darah EDTA simpan 2 jam 0,02-9,68 ng/mL, rerata 4,74 ng/mL. Ada perbedaan bermakna kadar cTnI darah EDTA segera diperiksa dengan plasma EDTA simpan 2 jam (p=.0,001), ), kadar cTnI sampel plasma EDTA simpan 2 jam menurun. Kata kunci : cTnI, darah EDTA, plasma, simpan 2 jam v THE LEVEL DIFFERENCE OF CARDIAC TROPONIN I (cTnI) EDTA BLOOD IMMEDIATELY CHECKED AND STORED FOR 2 HOURS Risdaryanti1, Fitri Nuroini2, Andri Sukeksi2 1. Study Program of D IV Health Analyst, Faculty of Nursing and Health, University of Muhammadiyah Semarang. 2. Clinical Pathology Laboratory Faculty of Nursing and Health, University of Muhammadiyah Semarang. ABSTRACT The examination of cardiac troponin I (cTnI) level requires a result with high accuracy and precision. The examination method of cTnI flourescence immunoassay using Point of Care Testing (POCT) tool with a waiting time or Turn Around Time (TATs) is less than 60 minutes. The complete blood specimens without or with efficient anticoagulants in sample preparation. Specimens must be processed and analyzed immediately because cardiac troponin is relatively unstable in the complete blood, so if stored it must be frozen. The specimen requirement for troponin is hemolysis-free plasma. The samples could be cooled or frozen if not checked immediately. Sometimes the clinician ask for confirmation of examination result, so that EDTA plasma is stored for a maximum of 2 hours at room temperature. The research aims to know the level difference of cTnI EDTA blood immediately checked and stored for 2 hours. The research type was analytic, cross sectional design. The research was done at RSUD Dr. Soewondo Kendal in June-July 2019. The research samples of 16 came from emergency room patients with IMA symptom. The examination material was EDTA blood. The examination using POCT cTnI. The research result of EDTA blood cTnI level which immediately checked 0,04-9,80 ng / mL, average 5,41 ng / mL. EDTA blood cTnI level which stored for 2 hours 0,02-9,68 ng / mL, average 4,74 ng / mL. There was difference in EDTA blood cTnI level immediately checked and stored for 2 hours (p = .0,001). Keywords: cTnI, EDTA blood, plasma, stored for 2 hours

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