PERBEDAAN KUALITAS JARINGAN TULANG PIPA TIKUS MENGGUNAKAN LARUTAN DEKALSIFIKASI ASAM NITRAT 3% DAN ASAM NITRAT 10% DENGAN PENGECATAN HE

Dewi,Yulia Ratna, G1C015038 (2019) PERBEDAAN KUALITAS JARINGAN TULANG PIPA TIKUS MENGGUNAKAN LARUTAN DEKALSIFIKASI ASAM NITRAT 3% DAN ASAM NITRAT 10% DENGAN PENGECATAN HE. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

ABSTRAK Dekalsifikasi yaitu proses meghilangkan garam kalsium pada tulang. Proses dekalsifikasi dapat menggunakan larutan asam seperti asam nitrat 3% dan asam nitrat 10%.Asam nitrat 3% membutuhkan waktu 8 hari dan kurang efektif sedangkan pada larutan asam nitrat 10% memerlukan waktu 2 sampai 3 hari dengan hasil dapat diagnosis dan kualitas preparat baik. Tujuan penelitian mengetahui perbedaan kualitas dan kelunakan jaringan tulang pipa tikus menggunakan larutan dekalsifikasi asam nitrat 3% dan asam nitrat 10% dengan pengecatan HE. Jenis penelitian adalah Analitik. Subjek penelitian menggunakan jaringan tulang pipa tikus wistar (Rattus norvegicus) normal yang berusia 2 sampai 3 bulan dengan jenis kelamin jantan. Objek penelitian menggunakan jaringan tulang pipa tikus putih jantan dengan galur Wistar sebayak 15 sediaan yang didekalsifikasi dengan larutan asam nitrat 3% dan 15 sediaan dengan larutan asam nitrat 10%. Hasil kelunakan jaringan tulang pipa tikus menggunakan larutan asam nitrat 10% lebih cepat lunak dibandingkan dengan asam nitrat 3%. Hasil kualitas preparat jaringan tulang pipa menggunakan asam nitrat 10% mendapatkan skor (1+) yaitu tidak dapat didiagnosis sedangkan asam nitrat 3% mendapatkan skor (3+) yaitu dapat didiagnosis. Simpulan penelitian terdapat perbedaan antara jaringan tulang pipa yang direndam dengan menggunakan larutan asam nitrat 3% dan asam nitrat 10%. Kata kunci: dekalsifikasi, asam nitrat 3%, asam nitrat 10%, tulang pipa tikus, dan HE   THE DIFFERENCES OF BONES TISSUE PIPE RAT QUALITY USING 3% DECALSIFICATION SOLUTION AND 10% NITRATIC ACID BY HE PAINTINGS Yulia Ratna Dewi1, Fitri Nuroini2, Arya Iswara2 1.Study Program D IV Health Analyst at the Faculty of Nursing and Health, Universitas Muhammdiyah Semarang 2.Sitohistotechnology Laboratory Faculty of Nursing and Health, Universitas Muhammadiyah Semarang ABSTRACT Decalcification is the process of removing calcium salts from bones. The decalcification process can use acidic solutions such as 3% nitric acid and 10% nitric acid. 3% nitric acid takes 8 days and is less effective while in 10% nitric acid solution takes 2 to 3 days with the results can be a good diagnosis and quality preparations.This study aimed to know the differences in the quality and softness of a pipe bone tissue using a decalcification solution of 3% nitric acid and 10% nitric acid with HE painting. This type of research is analytical. The subject of the research is pipe bone tissue Wistar rats (Rattus norvegicus) normal aged 2 until 3 months with the pipe bone tissue softness rats using 10% nitric acid solution faster than the 3% nitric acid. The results of the quality of pipe bone tissue preparations using 10% nitric acid get a score (1+) that can not be diagnosed while 3% nitric acid to get a score (3+) that can be diagnosed. The conclusions of the research there are a differences between the pipes bone tissue soaked with 3% solution of nitric acid and 10% nitric acid. male sex. The object of research is pipe bone tissue with a white male rats of Wistar 15 stocks were decalcified with 3% nitric acid solution and 15 samples with 10% nitric acid solution. The results of Keywords: decalcification, 3% nitric acid, 10% nitric acid, rat pipe bone, and HE

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