PERBEDAAN VARIASI WAKTU PEMBACAAN ABSORBANSI SETELAH PENAMBAHAN STOP SOLUTION TERHADAP KADAR HS-CRP PADA PENDERITA HIPERTENSI

Fitriyani,Anissatul, G1C218249 (2019) PERBEDAAN VARIASI WAKTU PEMBACAAN ABSORBANSI SETELAH PENAMBAHAN STOP SOLUTION TERHADAP KADAR HS-CRP PADA PENDERITA HIPERTENSI. Undergraduate thesis, Universitas Muhammadiyah Semarang.

[img] Text
Full Text.pdf
Restricted to Repository staff only

Download (2MB) | Request a copy

Abstract

ABSTRAK Enzim-linked Immunosorbent Assay (ELISA) merupakan metode pemeriksaan bidang imunologi yang digunakan untuk mendeteksi antigen dan antibodi dalam suatu sampel. Penambahan stop solution akan menghentikan sementara reaksi kompleks enzim-substrat. Aktivitas dan kesibukan yang tinggi di dalam laboratorium menyebabkan petugas laboratorium sering melakukan penundaan pembacaan. Adanya perbedaan variasi waktu pembacaan setelah penambah stop solution mendorong penulis untuk melakukan penelitian. Tujuan Penelitian ini adalah untuk mengetahui kadar hs-CRP pada penderita hipertensi dengan variasi waktu pembacaan absorbansi (segera, 15, dan, 30menit) pada ELISA reader setelah dilakukan penambahan stop solution. Penelitian ini merupakan studi eksperimental dengan pendekatan cross sectional. Sampel penelitian adalah penderita hipertensidi Puskesmas Halmahera Semarang. Analisis statistik dilakukan uji Kruskal-Wallis . Hasil pemeriksaan kadar hs-CRP pada waktu pembacaan segera yaitu sebesar 2,583 mg/L, menit ke 15 didapatkan hasil sebesar 2,573 mg/L, dan menit ke 30 didapatkan hasil sebesar 2,479 mg/L. Hasil analisis pada SPSS didapatkan nilai signifikansi atau probabilitas yaitu 0,558 (p > 0,05) sehingga diketahui bahwa tidak terdapat perbedaan yang bermakna antara variasi waktu pembacaan absorbansi metode ELISA terhadap kadar hs-CRP. Kata Kunci : ELISA, Hipertensi, hs-CRP   A Difference of Variation while Absorbance Reading Times After Addition Stop Solution to hs-CRP levels on Sufferer Hypertension Anissatul Fitriyani1, Budi Santosa2, Aprilia Indra Kartika1 1. DIV of Health AnalystStudi Program Nursing and Health Faculty Muhammadiyah University of Semarang 2. S2 of Master Program Sains Laboratorium Medis Muhammadiyah University of Semarang ABSTRACT Enzyme-Linked Immunosorbent Assay (ELISA) an immunological method used to detect antigents and antibodies in a sample. Adding of the stop solution will be stop the reaction of the enzyme-substatrate temporarily. High activity and busyness in the laboratory causes laboratory staff to often do the reading delay. The diffrence in reading time after addition stop solution encourages writer to do a research. The purpose of this research was to determine levels of hs-CRP on sufferer hypertension with variation while absorbance reading time (immediately, 15 and 30 minutes) in the ELISA reader after add stop solution. This reserach was an experimental study with cross sectional approach. The research sample is sufferer hypertension in the Puskesmas Halmahera Semarang. Statistical analysis was Kruskal-Wallis test. The result hs-CRP level at the time immediately is 2,583 mg/L, in the 15 minutes the result is 2,573 mg/L, and in the 30 minutes the result is 2,479 mg/L. SPSS analysis result ontained value significance or probability is 0,558 (p > 0,05) so it is known that there is no significance difference between variation while absorbance reading time to hs-CRP levels. Keywords : ELISA, Hypertension, hs-CRP

Item Type: Thesis (Undergraduate)
Call Number: 016/D4Ana/I/2020
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 13 Feb 2020 07:36
Last Modified: 13 Feb 2020 07:37
URI: http://repository.unimus.ac.id/id/eprint/3564

Actions (login required)

View Item View Item