PERBEDAAN GAMBARAN MIKROSKOPIS SEDIAAN JARINGAN HATI PADA PROSES CLEARING PEWARNAAN HEMATOXYLIN EOSIN (HE) MENGGUNAKAN BEBERAPA METODE (PERENDAMAN, PENCELUPAN DAN PENGGOYANGAN)

Hasmini, G1C218122 (2019) PERBEDAAN GAMBARAN MIKROSKOPIS SEDIAAN JARINGAN HATI PADA PROSES CLEARING PEWARNAAN HEMATOXYLIN EOSIN (HE) MENGGUNAKAN BEBERAPA METODE (PERENDAMAN, PENCELUPAN DAN PENGGOYANGAN). Undergraduate thesis, Universitas Muhammadiyah Semarang.

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Abstract

ABSTRAK Clearing adalah suatu proses dalam pewarnaan hemtoxylin eosin yang menggunakan metode perendaman, pencelupan dan penggoyangan untuk kualitas sediaan jaringan hati yang memberikan efek terhadap kejernihan dan transparan pada saat proses identifikasi. Tujuan Untuk mengetahui kualitas sediaan jaringan hati pada proses clearing menggunakan beberapa metode (perendaman, pencelupan dan penggoyangan). Gambaran mikroskopis sediaan jaringan hati proses clearing pewarnaan hemtoxylin eosin menggunakan metode perendaman di biarkan selama 10 menit, pencelupan diangkat dalam waktu 3 detik dilakukan selama 10 menit dan penggoyangan digoyangkan dengan bentuk angka 8 selama 10 menit. Hasil pengecatan hemtoxylin eosin pada proses clearing menggunakan metode perendaman diperoleh dari 10 preparat (100%) kategori baik (3), metode pencelupan diperoleh hasil dari 9 preparat (90%) kategori baik (3) dan 1 preparat (10%) kategori kurang baik (2), dan metode penggoyangan diperoleh hasil dari 8 preparat (80%) kategori baik (3) dan 2 preparat (20%) kategori kurang baik (2). Hasil uji statistik menggunakan uji kruskal-wallis diperoleh nilai p-value <0,05 yang menunjukan bahwa ada perbedaan gambaran mikroskopis sediaan jaringan hati normal hewan coba pada proses clearing dengan menggunakan metode perendaman, pencelupan dan penggoyangan pada sediaan jaringan. Hasil yang memiliki gambaran mikroskopis sediaan jaringan yang baik adalah metode perendaman dikarenakan sitoplasma dan inti sel terlihat jelas. kata kunci : Proses Clearing, Hematoxylin Eosin, Sediaan Jaringan Hati   DIFFERENCES IN QUALITY STOCKS IN THE PROCESS OF TISSUE HEART CLEARING STAINING HEMATOXYLIN EOSIN (HE) USING SOME METHOD (CROCHETERS MAN, DYEING AND ROCKING ) Hasmini 1 , Toeti Rahajoe 2 , Arya Iswara 2 1. Study Program D IV Health Analyst , Faculty of Nursing and Health, University of Muhammadiyah Semarang. 2. Laboratory Sitohistologi , Faculty Science of Nursing and Health University of Muhammadiyah Semarang ABSTRACT Clearing is a process in staining hemtoxylin eosin using the method of soaking , dyeing and shaking for the quality of liver tissue preparations that have an effect on clarity and transparency during the identification process . Objective To determine the quality of liver tissue preparation in the clearing process using several methods ( soaking , dipping and rocking ) . Quality stocks tissue careful process of clearing the coloring hemtoxylin eosin using methods marinade late on leave for 10 minutes , dyeing was appointed in time 3 seconds to do for 10 minutes and rocking shaken by form number 8 for 10 minutes . Results pengec a tan hemtoxylin eosin in the process of clearing using a method of soaking obtained from 10 preparations (100%) the category of either (3) , a method of dyeing obtained results of 9 preparations (90%) categories good (3) and 1 preparations (10%) categories less good (2) , and methods rocking obtained results from 8 preparations (80%) categories good (3) and 2 preparations (20%) categories of less well (2) . The results of the test statistic using the test Kruskal - Wallis obtained value p-value <0 , 05, which shows that there are differences in the quality of preparations of normal liver tissue of experimental animals in the process of clearing by using the immersion method, immersion and rocking on preparations network. keywords: Clearing Process , Hematoxylin Eosin , Liver Tissue Preparation

Item Type: Thesis (Undergraduate)
Call Number: 021/D4Ana/I/2020
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 13 Feb 2020 08:39
Last Modified: 13 Feb 2020 08:39
URI: http://repository.unimus.ac.id/id/eprint/3569

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