PERBEDAAN HASIL PEMERIKSAAN BILIRUBIN TOTAL SAMPEL SERUM, PLASMA EDTA DAN PLASMA HEPARIN

Reni, Ratna Trianggoro, G1C216119 (2017) PERBEDAAN HASIL PEMERIKSAAN BILIRUBIN TOTAL SAMPEL SERUM, PLASMA EDTA DAN PLASMA HEPARIN. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Pemeriksaan kimia klinik khususnya bilirubin sebaiknya menggunakan sampel serum, tetapi pada keadaan tertentu kadang menggunakan plasma. Pemeriksaan bilirubin sering menjadi permintaan pemeriksaan tambahan, namun sampel yang tersedia hanya plasma EDTA, atau plasma heparin. Tujuan penelitian ini untuk menganalisis perbedaan hasil pemeriksaan bilirubin total sampel serum, plasma EDTA dan plasma heparin. Metode penelitian adalah eksperimen, sampel penelitian adalah total populasi mulai tanggal 13 Juni-16 Juli 2017, sebanyak 30 sampel, diambil darah dan dipisah ke tabung serum, EDTA dan heparin, kemudian diperiksa kadar bilirubin totalnya. Analisa data menggunakan uji Kruskall Wallis setelah sebelumnya diuji normalitas menggunakan Shapiro Wilk, dan uji homogenitas menggunakan Levene Test. Hasil penelitian dari 30 sampel yang diteliti, kadar bilirubin total dengan menggunakan sampel serum memiliki rata-rata sebesar 9,531 mg/dl; plasma EDTA memiliki rata-rata sebesar 8,718 mg/dl; dan plasma heparin rata-rata sebesar 9,462 mg/dl. Berdasarkan uji beda menggunakan Kruskall Wallis menunjukkan bahwa nilai tingkat signifikan Kruskall Wallis sebesar 0,806 > nilai α = 0,05. Berdasarkan analisis hasil penelitian, dinyatakan bahwa tidak terdapat perbedaan bermakna pengukuran kadar bilirubin total menggunakan sampel serum, plasma EDTA dan plasma heparin. Hasil penelitian ini menunjukkan bahwa kadar bilirubin total dapat diukur menggunakan sampel serum, plasma EDTA, dan plasma heparin. Kata Kunci : Bilirubin Total, Serum, Plasma EDTA, Plasma Heparin. Clinical chemistry examination especially bilirubin should use serum sample, but in certain circumstances sometimes use plasma. Examination of bilirubin often becomes a demand for additional examination, where as only samples of EDTA plasma, or plasma heparin. The purpose of this study was to analyze the difference of bilirubin examination result of total serum sample, EDTA plasma and heparin plasma. The research method is experiment, the research sample is total population starting from June 13 to July 16, 2017, as many as 30 samples, taken blood and split into serum tubes, EDTA and heparin, then checked the total bilirubin levels. Data analysis using Kruskall Wallis test after previously tested normality using Shapiro Wilk, and homogeneity test using Levene Test. Results of the study of 30 samples studied, total bilirubin content using serum samples had an average of 9.531 mg / dl; EDTA plasma has an average of 8.718 mg / dl; And an average plasma heparin of 9,462 mg / dl. Based on different test using Kruskall Wallis shows that the value of significant level of Kruskall Wallis equal to 0,806> value of α = 0,05. Based on the analysis of the results of the study, it was stated that there was no significant difference in the measurement of total bilirubin levels using serum samples, EDTA plasma and plasma heparin. The results of this study indicate that total bilirubin levels can be measured using serum samples, EDTA plasma, and heparin plasma Keywords: Total Bilirubin, Serum, EDTA Plasma, Heparin Plasma.

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