PENGARUH PENUNDAAN DAN VOLUME DARAH EDTA TERHADAP PEMERIKSAAN KADAR HEMATOKRIT METODE MIKRO

Andriyani, Sega Fajar, G0C015033 (2018) PENGARUH PENUNDAAN DAN VOLUME DARAH EDTA TERHADAP PEMERIKSAAN KADAR HEMATOKRIT METODE MIKRO. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Hematocrit examination is one of the special blood tests that are often done in the laboratory to help diagnose various diseases including Dengue Hemorrhagic Fever (DHF), anemia, polycythemia vera and severe diarrhea. Hematocrit levels can also be used to calculate average erythrocyte values. One of the factors that can influence the examination of hematocrit, namely delaying examination of EDTA samples can cause a series of erythrocyte changes such as constriction of erythrocytes causing low hematocrit values.The study was conducted to determine whether there is an effect of EDTA volume and sample storage time on hematocrit levels. The examination was conducted to determine the effect of micro-hematocrit levels on EDTA blood 10 ul and 50 ul which were examined immediately, delayed 1 hour and 2 hours at room temperature. This type of research is analytical research. The sample was taken randomly as many as 9 students from a total population of 94 students in the sixth semester DIII Health Analyst at Muhammadiyah University Semarang. The results of the examination showed the average examination of 10 ul hematocrit levels at immediate examination, a delay of 1 hour and 2 hours from the examination showed an average of 43.44, 39.67, and 36.56, while the average examination of hematocrit levels was 50 ul on immediate inspection, a delay of 1 hour and 2 hours is 41.00, 37.00, 35.78. One Way Anova test shows a significance value of 0.033 with a significance level of 0.033 <0.05. It can be concluded that there is an effect of delay and EDTA blood volume on the examination of micro-hematocrit levels. Keyword : Volume Effect, Effect Time, Hematocrit Levels

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