DETEKSI GEN Coa PADA METHICILLIN RESISTANT Staphylococcus aureus (MRSA)

Rosalina, Zainab, G1C217019 (2018) DETEKSI GEN Coa PADA METHICILLIN RESISTANT Staphylococcus aureus (MRSA). Undergraduate thesis, Universitas Muhammadiyah Semarang.

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Abstract

Tujuan dari penelitian ini untuk mendeteksi gen Coa pada Methicillin Resistant Staphylococcus aureus (MRSA). Sampel dalam penelitian ini adalah empat sampel bakteri MRSA (Sa1, Sa2, Sa3, Sa4). Deteksi gen Coa : 1) Isolasi DNA bakteri MRSA dengan metode Chelating Ion Exchange Resin (Chelex). 2) Amplifikasi gen Coa dengan metode PCR menggunakan primer spesifik Forward (5´- ATAGAGATGCTGGTACAGG-´3) dan primer Reverse (5´GCTTCCGATTGTTCGATGC-3´). 3) Visualisasi hasil PCR dengan metode Elektroforesis gel Agarose. Hasil penelitian menunjukkan bahwa empat sampel bakteri MRSA (Sa1, Sa2, Sa3, Sa4) positif memiliki gen Coa yang berukuran 756 bp. Kata kunci : Methicillin Resistant Staphylococcus aureus (MRSA), Gen Coa

Item Type: Thesis (Undergraduate)
Call Number: 196/D4.Ana/1/2019
Contributors Thesis: 1. Dr. Sri Darmawati, M.Si 2. Muhammad Evy Prastiyanto, M.Sc
Subjects: R Medicine > Health Analyst
Divisions: Faculty of Nursing and Health > D4 Health Analyst
Depositing User: perpus unimus
Date Deposited: 18 Mar 2019 07:34
Last Modified: 18 Mar 2019 07:34
URI: http://repository.unimus.ac.id/id/eprint/3087

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