Wahyuni, Rizka Ayu, G1C216210 (2017) DETEKSI GEN Coa PADA Staphylococcus aureus YANG DI ISOLASI DARI SUSU SAPI MURNI. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].
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Abstract
Tujuan penelitian ini untuk mendeteksi gen Coa pada S. aureus yang diisolasi dari susu sapi murni di Kelurahan Gedawang. Jenis penelitian ini adalah penelitian deskriptif. Bakteri S. aureus diisolasi dari 15 sampel susu sapi murni. Deteksi gen Coa menggunakan primer spesifik Forward (5´- ATAGAGATGCTGGTACAGG-´3) dan primer Coa Reverse (5´GCTTCCGATTGTTCGATGC-3´). Jumlah isolat S. aureus yang didapatkan dari isolasi bakteri sebanyak 3 sampel positif yang memiliki gen Coa dengan hasil produk 756 bp. Hasil pada penelitian ini menunjukkan hasil positif gen Coa sebesar 100%. Kata kunci : Staphylococcus aureus, Susu, gen Coa The purpose of this research is to detect the genes of the Coa on S. aureus was isolated from pure cow’s milk in Kelurahan Gedawang. Type of this research is descriptive. The bacteria S. aureus isolated from 15 samples of pure cow’s milk. Detection of a gene specific primer using Coa. Forward (5´- ATAGAGATGCTGGTACAGG-´3) and primer Coa Reverse (5´GCTTCCGATTGTTCGATGC-3´). The number of S. aureus isolates obtained from the isolation of bacteria as many as three positive samples had genes with Coa product results 756 bp. The results in this study showed a positive of 100% Coa gen. Keyword: Staphylococcus aureus, milk, gene Coa
| Item Type: | Thesis (Sarjana / Sarjana Terapan (S1/D4) ) |
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| Contributors Thesis: | 050/D4Ana/XI/2017 |
| Subjects: | R Medicine > Health Analyst |
| Divisions: | Faculty of Nursing and Health > D4 Health Analyst |
| Depositing User: | perpus unimus |
| URI: | http://repository.unimus.ac.id/id/eprint/1271 |
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