PERBEDAAN JUMLAH RETIKULOSIT TERHADAP PENYIMPANAN DARAH EDTA PADA PASIEN ANEMIA

Sulistyaningsih, Dyah, G1C216222 (2017) PERBEDAAN JUMLAH RETIKULOSIT TERHADAP PENYIMPANAN DARAH EDTA PADA PASIEN ANEMIA. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Reticulocyte count is used to assess the accuracy of bone marrow reaction towards anemia. The relatively accurate reticulocyte count helps to determine the anemia type. Examination material of capillary blood or venous blood with EDTA anticoagulant. EDTA blood storage needs to pay attention to the sample stability. Temperature and storage duration may affect the examination result. The 4ºC temperature of EDTA blood had not change the cell morphology. The reticulocyte amount examination is using EDTA blood samples should be performed quickly or less than 1 hour after taking. The problem that occurred, patient's examination was came on afternoon delayed until the next day, estimated to be delayed for 12 hours. The research goal is to know the difference of EDTA blood’s reticulocyte amount towards EDTA blood storage on anemia patients. The research type is analytical with cross sectional approach. Reticulocyte examination was using dry preparation method. Reticulocyte amount 1 hour after taking was 0.10-7.90 and average was 2.51. Reticulocyte amount after 12 hours of storage, with 4ºC temperature, 0.10-7,70, and average was 2.70. Statistic test with Wilcoxon was obtained p > 0,05 which means there was no significant difference on reticulocyte amount 1 hour after taking with 12 hours of storage with 4ºC temperature. Keywords : reticulocyte, storage

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