Identifikasi Gen mecA pada Methicillin-resistant Staphylococcus aureus

Mindhumalid, Tatut, G1C014039 (2018) Identifikasi Gen mecA pada Methicillin-resistant Staphylococcus aureus. Sarjana / Sarjana Terapan (S1/D4) thesis, ["eprint_fieldopt_institution_Universitas Muhammadiyah Semarang" not defined].

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Abstract

Methicillin-resistant Stapylococcus aureus (MRSA) is caused by the resistant gene encoding, mecA. The mecA gene is located inside the SCCmec and it also produces PBP2a. Nowadays, methicillin is not produced commercially anymore, so the treatment is replacing by using oxacillin, still one group of -lactams. The purpose of this study is to identify the presence of mecA genes in MRSA. The method used in this study is the sensitivity test of Oxacillin disk and detection of presence of the mecA gene using PCR against 4 S. aureus isolates (Sa1, Sa2, Sa3 and Sa4) derived from clinical samples. The results showed that of 4 S. aureus isolates, 3 of them were Sa1, Sa2, and Sa3 resistant to oxacillin and showed the positive results in the presence of mecA gene with a product of 533 bp. Meanwhile Sa4 is sensitive to Oxacillin and showed the negative results in the presence of the mecA gene. It can be concluded that there is mecA gene on MRSA. Keyword : MRSA, Oxacillin, mecA gene

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